Addgene - pMKInt-hyg Plasmid Data

Plasmid 18953: pMKInt-hyg

Gene/insert name:
phiC31 integrase
Insert size:
1874
Species:
Streptomyces phage phiC31
Entrez Gene:
int ()
Vector backbone:
pMK33
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
4100
Cloning site 5':
EcoRV
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRV
Site destroyed during cloning:
Yes
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pIJ8600 (Mervyn Bibb, John Innes Institute, Norwich, U.K.)
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Michele Calos
Terms and Licenses:
MTA

Comments:

Map of parent vector is uploaded

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Groth et al (Genetics. 2004 Apr . 166(4):1775-82. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18953" in your Materials and Methods section.