|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18953||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeInsect Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namephiC31 integrase
SpeciesStreptomyces phage phiC31
Insert Size (bp)1874
Entrez Geneint (a.k.a. phiC31p51)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypIJ8600 (Mervyn Bibb, John Innes Institute, Norwich, U.K.)
Terms and Licenses
Map of parent vector is uploaded
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMKInt-hyg was a gift from Michele Calos (Addgene plasmid # 18953 ; http://n2t.net/addgene:18953 ; RRID:Addgene_18953)
For your References section:Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Groth AC, Fish M, Nusse R, Calos MP. Genetics. 2004 Apr . 166(4):1775-82. 10.1534/genetics.166.4.1775 PubMed 15126397
Map uploaded by the depositor.