pHIV-Wnt1HA
(Plasmid #18983)

• Depositing Labs: Bryan Welm, Zena Werb
• Publication: Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102.

Backbone

• Vector backbone: HIV-Zsgreen
• Backbone size w/o insert (bp): 7664
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Growth in Stbl3 is preferred
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: wingless-related MMTV integration site 1
• Alt name: Wnt1
• Alt name: Mouse int-1 proto-oncogene
• Species: M. musculus (mouse)
• Insert Size (bp): 1100
• GenBank ID: M11943
• Entrez Gene: Wnt1 (a.k.a. Int-1, Wnt-1, sw, swaying)
• Tag / Fusion Protein:
  • HA-Tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: 5’-TGGAATTTGCCCTTTTTGAG-3’
• 3′ sequencing primer: 5’-AGGAACTGCTTCCTTCACGA-3’

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Fung,Y.K., Shackleford,G.M., Brown,A.M., Sanders,G.S. and Varmus,H.E. Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene. Mol. Cell. Biol. 5 (12), 3337-3344 (1985)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Wnt1HA insert was cloned into the XbaI site of HIV-Zsgreen in order to generate a bicistronic lentivirus that coexpresses Wnt1HA with Zsgreen under the control of the EF1alpha promoter. Gerrit Dijkgraaf in Zena Werb's lab cloned this virus.