Addgene - pGEX-2TK-YAP-Ag Plasmid Data

Plasmid 19063: pGEX-2TK-YAP-Ag

Gene/insert name:
Yes-kinase associated protein
Alt name:
YAP2, YAP65
Alt name:
YAP2 isoform
Insert size:
444
Species:
H. sapiens (human)
GenBank ID:
NM_006106
Entrez Gene:
YAP1 (YAP, YAP2, YAP65, YKI)
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Vector backbone:
pGEX-2TK
(Search Vector Database)
Backbone manufacturer:
Pharmacia
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4969
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
N/A List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Marius Sudol
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mst2 and Lats kinases regulate apoptotic function of YAP. Oka et al (J Biol Chem. 2008 Jul 17. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19063" in your Materials and Methods section.