pLenti PGK Blast DEST (w524-1)
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size (bp) 9313
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsAlso resistant to Chloramphinecol. DB3.1 or ccDB survival, 37oC
Copy numberHigh Copy
- Cloning method Gateway Cloning
- 5′ sequencing primer hPGK-F (5'-GTGTTCCGCATTCTGCAAG-3') (Common Sequencing Primers)
Terms and Licenses
Article Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti PGK Blast DEST (w524-1) was a gift from Eric Campeau (Addgene plasmid # 19065)
For your References section:A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.