pMIR-D10 mutUTR
(Plasmid #19116)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pMIR-report
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 6470
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    HOXD10 mut 3' UTR
  • Alt name
    homeobox D10
  • Alt name
    HOX4; HOX4D; HOX4E; Hox-4.4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    810
  • Entrez Gene
    HOXD10 (a.k.a. HOX4, HOX4D, HOX4E, Hox-4.4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer Luc-F
  • 3′ sequencing primer M13-F20
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Has a substitution of 4 nucleotides within the mir-10b binding site: from TCGTAATGCAGGGTA in wt to TCGTAATGCACCCAA in mutant

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIR-D10 mutUTR was a gift from Bob Weinberg (Addgene plasmid # 19116)
  • For your References section:

    Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Ma L, Teruya-Feldstein J, Weinberg RA. Nature. 2007 Oct 11. 449(7163):682-8. 10.1038/nature06174 PubMed 17898713