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Plasmid 19116: pMIR-D10 mutUTR
  • HOXD10 mut 3' UTR

  • homeobox D10

  • HOX4; HOX4D; HOX4E; Hox-4.4

  • 810

  • H. sapiens (human)

  • HOXD10 (HOX4, HOX4D, HOX4E, Hox-4.4)

  • pMIR-report
    (Search Vector Database)

  • Ambion

  • Mammalian Expression

  • 6470

  • SpeI

  • No

  • HindIII

  • No

  • Luc-F List of Sequencing Primers

  • M13-F20

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (1)
  • Bob Weinberg

  • UBMTA

Comments: 

Has a substitution of 4 nucleotides within the mir-10b binding site: from TCGTAATGCAGGGTA in wt to TCGTAATGCACCCAA in mutant

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Ma et al (Nature. 2007 Oct 11. 449(7163):682-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19116" in your Materials and Methods section.