Addgene - pMIR-D10 mutUTR Plasmid Data

Plasmid 19116: pMIR-D10 mutUTR

Gene/insert name:
HOXD10 mut 3' UTR
Alt name:
homeobox D10
Alt name:
HOX4; HOX4D; HOX4E; Hox-4.4
Insert size:
810
Species:
H. sapiens (human)
Entrez Gene:
HOXD10 (HOX4, HOX4D, HOX4E, Hox-4.4)
Vector backbone:
pMIR-report
(Search Vector Database)
Backbone manufacturer:
Ambion
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6470
Cloning site 5':
SpeI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
Luc-F List of Sequencing Primers
3' sequencing primer:
M13-F20
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Has a substitution of 4 nucleotides within the mir-10b binding site: from TCGTAATGCAGGGTA in wt to TCGTAATGCACCCAA in mutant

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Ma et al (Nature. 2007 Oct 11. 449(7163):682-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19116" in your Materials and Methods section.