|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19116||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6470
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHOXD10 mut 3' UTR
Alt namehomeobox D10
Alt nameHOX4; HOX4D; HOX4E; Hox-4.4
SpeciesH. sapiens (human)
Insert Size (bp)810
Entrez GeneHOXD10 (a.k.a. HOX4, HOX4D, HOX4E, Hox-4.4)
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer Luc-F
- 3′ sequencing primer M13-F20 (Common Sequencing Primers)
Has a substitution of 4 nucleotides within the mir-10b binding site: from TCGTAATGCAGGGTA in wt to TCGTAATGCACCCAA in mutant
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIR-D10 mutUTR was a gift from Bob Weinberg (Addgene plasmid # 19116)
For your References section:Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Ma L, Teruya-Feldstein J, Weinberg RA. Nature. 2007 Oct 11. 449(7163):682-8. 10.1038/nature06174 PubMed 17898713
Generated by Addgene from plasmid data.