shp63alpha pLKO.1 puro
(Plasmid #19120)

• Depositing Lab: Bob Weinberg
• Publication: Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Backbone

• Vector backbone: pLKO.1 puro
• Backbone manufacturer: GenHunter
• Backbone size w/o insert (bp): 7032
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use Stbl3 or HB101 to reduce chance of recombination.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: small hairpin RNA against tumor protein p63
• Alt name: p63, AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p73H, p73L
• Species: H. sapiens (human)
• Entrez Gene: TP63 (a.k.a. AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LKO.1 5'

Resource Information

• Reference:
  • Addgene's pLKO.1 protocol
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

shRNA against p63 (all isoforms), cloned by Watnick, RS.
shRNA sequence - 5'-CCG GCC GTT TCG TCA GAA CAC ACA TCT CGA GAT GTG TGT TCT GAC GAA ACG
GTT TTT