pCMV BAF170-Flag
(Plasmid #19142)

• Depositing Lab: Joan Massague
• Publication: Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4657
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
• Alt name: SMARCC2, BAF170, CRACC2, Rsc8
• Species: H. sapiens (human)
• Insert Size (bp): 4000
• Entrez Gene: SMARCC2 (a.k.a. BAF170, CRACC2, CSS8, Rsc8)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SphI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Hong-van Le
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

hunam BAF170 cDNA from pFASTBAC-BAF170 Kingston's lab