Addgene - pRK5-HA GST RagA wt Plasmid Data

Plasmid 19298: pRK5-HA GST RagA wt

Gene/insert name:
Ras-related GTP binding A
Alt name:
RRAGA
Insert size:
1610
Species:
H. sapiens (human)
Entrez Gene:
RRAGA (FIP-1, FIP1, RAGA)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Fusion protein or tag:
GST
Terminal:
N terminal on insert
Vector backbone:
pRK5
(Search Vector Database)
Backbone manufacturer:
BD PharMingen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4754
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
SP6, pGEX 5' List of Sequencing Primers
3' sequencing primer:
pBABE 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al (Science. 2008 Jun 13. 320(5882):1496-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19298" in your Materials and Methods section.