pTREtight2-HA_GDP1
(Plasmid
#19408)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19408 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTREtight2
- Backbone size w/o insert (bp) 2932
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5a, 37°C
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGDP1
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Alt nameKLLA0F09141g hypothetical protein
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SpeciesKluyveromyces lactis
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Insert Size (bp)1071
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GenBank IDAJ430565
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Entrez GeneKLLA0F09141g
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTREtight2-HA_GDP1 was a gift from Markus Ralser (Addgene plasmid # 19408 ; http://n2t.net/addgene:19408 ; RRID:Addgene_19408)
Map uploaded by the depositor.
