Addgene - pBABE BRG1 Plasmid Data

Plasmid 1959: pBABE BRG1

Gene/insert name:
BRG1
Alt name:
BAF190a
Insert size:
5000
Species:
H. sapiens (human)
Entrez Gene:
SMARCA4 (BAF190, BAF190A, BRG1, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b)
Fusion protein or tag:
flag
Terminal:
C terminal on insert
Vector backbone:
pBABE-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5200
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Robert Kingston
Terms and Licenses:
MTA

Comments:

Vector: pBabe linearized with SalI and CIP'd. Insert: pBS-CeBRG1 digested with SalI to liberate entire flag tagged BRG1 seq. (Kingston #1359)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes. Sif et al (Genes Dev 2001 Mar 1;15(5):603-18. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1959" in your Materials and Methods section.