pBABE hBRM
(Plasmid #1961)

• Depositing Lab: Robert Kingston
• Publication: Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BRM
• Alt name: BAF190b
• Species: H. sapiens (human)
• Entrez Gene: SMARCA2 (a.k.a. BAF190, BIS, BRM, NCBRS, SNF2, SNF2L2, SNF2LA, SWI2, Sth1p, hBRM, hSNF2a)
• Tag / Fusion Protein:
  • flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector: pBabe linearized with EcoRI and CIP'd. Insert: pBS-hBRM digested with EcoRI to liberate entire flag tagged BRM seq. (Kingston #1360)