|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19689||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeWorm Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert namehlh-12(R15K)_genomic region
SpeciesC. elegans (nematode)
- Cloning method TOPO Cloning
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGC85 was a gift from Jane Hubbard (Addgene plasmid # 19689 ; http://n2t.net/addgene:19689 ; RRID:Addgene_19689)
For your References section:A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. Voutev R, Hubbard EJ. Genetics. 2008 Sep . 180(1):103-19. 10.1534/genetics.108.090274 PubMed 18723890