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pXJ40-HA-Merlin I S518A
(Plasmid #19700)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19700 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pXJ40-HA
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Merlin I
  • Alt name
    Nf2 neurofibromatosis 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1800
  • Mutation
    Serine 518 to Alanine
  • GenBank ID
    P46662
  • Entrez Gene
    Nf2 (a.k.a. merlin)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXJ40-HA-Merlin I S518A was a gift from Filippo Giancotti (Addgene plasmid # 19700 ; http://n2t.net/addgene:19700 ; RRID:Addgene_19700)
  • For your References section:

    Merlin/NF-2 mediates contact inhibition of growth by suppressing recruitment of Rac to the plasma membrane. Okada T, Lopez-Lago M, Giancotti FG. J Cell Biol. 2005 Oct 24. 171(2):361-71. 10.1083/jcb.200503165 PubMed 16247032