Addgene - pXJ40-HA-Merlin I S518A Plasmid Data

Plasmid 19700: pXJ40-HA-Merlin I S518A

Gene/insert name:
Merlin I
Alt name:
Nf2 neurofibromatosis 2
Insert size:
1800
Species:
M. musculus (mouse)
GenBank ID:
P46662
Entrez Gene:
Nf2 (RP23-280J3.6, merlin)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Mutation:
Serine 518 to Alanine
Vector backbone:
pXJ40-HA
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5000
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
KpnI
Site destroyed during cloning:
Unknown
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (3)
Supplemental document:
pXJ40-HA (application/pdf)
Principal Investigator:
Filippo Giancotti
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Merlin/NF-2 mediates contact inhibition of growth by suppressing recruitment of Rac to the plasma membrane. Okada et al (J Cell Biol. 2005 Oct 24. 171(2):361-71. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19700" in your Materials and Methods section.