pCDNA3 Flag MKK7B2Jnk3a2
(Plasmid #19729)

• Depositing Lab: Roger Davis
• Publication: Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MKK7B2Jnk3a2
• Alt name: MAP2K7
• Alt name: Jnk3
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 2698
• Entrez Gene: Map2k7 (a.k.a. 5930412N11Rik, JNKK 2, Jnkk, Jnkk2, MAPKK 7, MEK 7, Mapkk7, Mek7, Mkk7, Prkm, Prkmk7, sek, sek2)
• Entrez Gene: MAPK10 (a.k.a. JNK3, JNK3A, PRKM10, SAPK1b, p493F12, p54bSAPK)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Sma I (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry