MSCV FLIPi P2G_Thy1.1 p53
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19748||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneMSCV FLIPi
- Backbone size w/o insert (bp) 7994
Vector typeMammalian Expression, Retroviral, RNAi, Cre/Lox
Growth in Bacteria
Growth instructionsgrow in stbl3 cells at 30oC
Gene/Insert nameoligo targeting p53
SpeciesM. musculus (mouse)
Insert Size (bp)387
Entrez GeneTrp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer GFP miR primer (GCTGGAGTTCGTGACCGCC) (Common Sequencing Primers)
Constitutively expresses puromycin-resistance and GFP. Recombines to express surface Thy1.1 and miR30-based RNAi. The miRNA is contained within an artificial intron, and splicing of the intron can boost transgene expression (1.5 to 2-fold). The expression of surface Thy1.1 facilitates antibody-mediated cell sorting.
See FLIP vector protocols (PDF from this page) for more information.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV FLIPi P2G_Thy1.1 p53 was a gift from Richard Hynes (Addgene plasmid # 19748)
For your References section:A system for Cre-regulated RNA interference in vivo. Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO. Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. 10.1073/pnas.0806907105 PubMed 18779577
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.