MSCV P2Gm FF
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19750||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneMSCV P2Gm
Backbone manufacturerMSCV2.2 with WPRE cloned into ClaI site is the base vector
- Backbone size w/o insert (bp) 7518
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Growth instructionsgrow in stbl3 cells at 30oC
Gene/Insert nameoligo targeting Firefly luciferase
Insert Size (bp)100
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer GFP miR primer (GCTGGAGTTCGTGACCGCC) (Common Sequencing Primers)
MSCV-based entry vector for cloning and validating miR30-based RNAi constructs. The vector expresses puromycin-resistance and GFP, separated by the FMDV 2A peptide.
This is considered the "empty" vector and can be used for cloning. It contains a hairpin targeting Firefly luciferase.
See FLIP vector protocols (PDF from this page) for more information.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV P2Gm FF was a gift from Richard Hynes (Addgene plasmid # 19750)
For your References section:A system for Cre-regulated RNA interference in vivo. Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO. Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. 10.1073/pnas.0806907105 PubMed 18779577