Addgene - pLB2 CAG P2Gm Plasmid Data

Plasmid 19752: pLB2 CAG P2Gm

Gene/insert name:
None
Vector backbone:
LB2
(Search Vector Database)
Vector type:
Mammalian Expression, Mouse Targeting, Lentiviral, RNAi
Backbone size (bp):
10552
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
PmeI
Site destroyed during cloning:
No
5' sequencing primer:
GFP miR primer (GCTGGAGTTCGTGACCGCC) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
Growth instructions:
grow in stbl3 cells at 30oC
High or low copy:
Low Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Supplemental document:
FLIP vector protocols (application/pdf)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Richard Hynes
Terms and Licenses:
MTA

Comments:

Lentiviral vector that constitutively expresses puromycin-resistance and GFP driven by the CAGGS promoter.

See FLIP vector protocols (PDF from this page) for more information.

Firefly hairpin sequence is -
AAGGTATATTGCTGTTGACAGTGAGCGAGCTCCCGTGAATTGGAATCCTA
GTGAAGCCACAGATGTAGGATTCCAATTCAGCGGGAGCCTGCCTACTGCC
TCG

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A system for Cre-regulated RNA interference in vivo. Stern et al (Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19752" in your Materials and Methods section.