pLB2 CAG P2Gm
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19752||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 10552
Vector typeMammalian Expression, Mouse Targeting, Lentiviral, RNAi
Growth in Bacteria
Growth instructionsgrow in stbl3 cells at 30oC
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer GFP miR primer (GCTGGAGTTCGTGACCGCC) (Common Sequencing Primers)
Lentiviral vector that constitutively expresses puromycin-resistance and GFP driven by the CAGGS promoter.
See FLIP vector protocols (PDF from this page) for more information.
Firefly hairpin sequence is -
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLB2 CAG P2Gm was a gift from Richard Hynes (Addgene plasmid # 19752 ; http://n2t.net/addgene:19752 ; RRID:Addgene_19752)
For your References section:A system for Cre-regulated RNA interference in vivo. Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO. Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. 10.1073/pnas.0806907105 PubMed 18779577