Addgene - pLB2 Ubi-FLIP FF Plasmid Data

Plasmid 19753: pLB2 Ubi-FLIP FF

Gene/insert name:
oligo targeting Firefly luciferase
Insert size:
386
Vector backbone:
LB2 FLIP
(Search Vector Database)
Vector type:
Mammalian Expression, Mouse Targeting, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
10463
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
PmeI
Site destroyed during cloning:
No
5' sequencing primer:
GFP miR primer (GCTGGAGTTCGTGACCGCC) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
Growth instructions:
grow in stbl3 cells at 30oC
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Supplemental document:
FLIP vector protocols (application/pdf)
Principal Investigator:
Richard Hynes
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Lentiviral vector that constitutively expresses puromycin-resistance and surface Thy1.1 driven by the UbiquitinC promoter. Recombines to express GFP and miR30-based RNAi.

See FLIP vector protocols (PDF from this page) for more information.

There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A system for Cre-regulated RNA interference in vivo. Stern et al (Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19753" in your Materials and Methods section.