pLB2 Ubi-FLIP FF
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19753||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneLB2 FLIP
- Backbone size w/o insert (bp) 10463
Vector typeMammalian Expression, Mouse Targeting, Lentiviral, RNAi, Cre/Lox
Growth in Bacteria
Growth instructionsgrow in stbl3 cells at 30oC
Gene/Insert nameoligo targeting Firefly luciferase
Insert Size (bp)386
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer GFP miR primer (GCTGGAGTTCGTGACCGCC) (Common Sequencing Primers)
Lentiviral vector that constitutively expresses puromycin-resistance and surface Thy1.1 driven by the UbiquitinC promoter. Recombines to express GFP and miR30-based RNAi.
See FLIP vector protocols (PDF from this page) for more information.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLB2 Ubi-FLIP FF was a gift from Richard Hynes (Addgene plasmid # 19753)
For your References section:A system for Cre-regulated RNA interference in vivo. Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO. Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. 10.1073/pnas.0806907105 PubMed 18779577