Addgene - human STIM1 YFP Plasmid Data

Plasmid 19754: human STIM1 YFP

Gene/insert name:
STIM1
Alt name:
stromal interaction molecule 1
Species:
H. sapiens (human)
Entrez Gene:
STIM1 (D11S4896E, GOK, STIM1L, TAM)
Fusion protein or tag:
YFP
Terminal:
C terminal on insert
Vector backbone:
MO91
(Search Vector Database)
Backbone manufacturer:
See author's map
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6000
Cloning site 5':
XhoI
Site destroyed during cloning:
Unknown
Cloning site 3':
NotI
Site destroyed during cloning:
Unknown
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Anjana Rao
Terms and Licenses:
MTA

Comments:

See Author's Map for empty backbone (MO91) information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Orai1 is an essential pore subunit of the CRAC channel. Prakriya et al (Nature. 2006 Sep 14. 443(7108):230-3. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19754" in your Materials and Methods section.