1
Plasmid 19765: pLV-tetO-Sox2
  • Sox2

  • 950

  • M. musculus (mouse)

  • Sox2 (AL606746.1, Sox-2, lcc, ysb)

  • FUdeltaGW
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 9941

  • EcoRI

  • No

  • EcoRI

  • No

  • CMV-F List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • Stbl3

  • High Copy

  • View sequences (2)
  • View map

  • Konrad Hochedlinger

  • UBMTA

Comments: 

From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Stadtfeld et al (Cell Stem Cell. 2008 Mar 6. 2(3):230-40. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19765" in your Materials and Methods section.