psiCHECK RBJ 3'UTR
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19866||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6249
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRBJ 3'UTR
SpeciesH. sapiens (human)
Insert Size (bp)3957
Entrez GeneDNAJC27 (a.k.a. RBJ, RabJS, DKFZp434N211)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer GTACATCAAGAGCTTCGTGG
- 3′ sequencing primer AAGACTCATTTAGATCCTCACAC (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psiCHECK RBJ 3'UTR was a gift from Thomas Tuschl (Addgene plasmid # 19866)
For your References section:Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, Ojo T, Hafner M, Zavolan M, Tuschl T. RNA. 2008 Oct 31. ():. 10.1261/rna.1351608 PubMed 18978028
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.