Addgene - psiCHECK RBJ 3'UTR Plasmid Data

Plasmid 19866: psiCHECK RBJ 3'UTR

Gene/insert name:
RBJ 3'UTR
Alt name:
DNAJC27
Insert size:
3957
Species:
H. sapiens (human)
GenBank ID:
NM_016544
Entrez Gene:
DNAJC27 (RBJ, RabJS, DKFZp434N211)
Vector backbone:
psiCHECK-2
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6249
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
GTACATCAAGAGCTTCGTGG List of Sequencing Primers
3' sequencing primer:
AAGACTCATTTAGATCCTCACAC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Thomas Tuschl
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. Landthaler et al (RNA. 2008 Oct 31. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19866" in your Materials and Methods section.