HA-Elongin B-pcDNA3.1(+)-Zeo
(Plasmid #20000)

• Depositing Lab: William Kaelin
• Publication: Ohh et al Nat Cell Biol. 2000 Jul . 2(7):423-7.

Backbone

• Vector backbone: pcDNA3.1(+)-Zeo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5022
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Bleocin (Zeocin), 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha or XL1 @ 37˚C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: transcription elongation factor B
• Alt name: Elongin B
• Alt name: TCEB2
• Species: H. sapiens (human)
• Insert Size (bp): 367
• Mutation: wild type
• GenBank ID: NM_007108
• Entrez Gene: ELOB (a.k.a. SIII, TCEB2)
• Tag / Fusion Protein:
  • HA-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry