RelA cFlag pcDNA3
(Plasmid #20012)

• Depositing Lab: Stephen Smale
• Publication: Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Backbone

• Vector backbone: cFlag pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5-alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RelA
• Alt name: p65
• Species: M. musculus (mouse)
• Insert Size (bp): 1650
• GenBank ID: NM_009045
• Entrez Gene: Rela (a.k.a. p65, p65 NF-kappa B, p65 NFkB)
• Tag / Fusion Protein:
  • Flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 35 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See cFlag pcDNA plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).