Addgene - p50 cFlag pcDNA3 Plasmid Data

Plasmid 20018: p50 cFlag pcDNA3

Gene/insert name:
Nfkb1
Alt name:
p50
Insert size:
1284
Species:
M. musculus (mouse)
Entrez Gene:
Nfkb1 (NF-KB1, NF-kappaB, NF-kappaB1, p105, p50, p50/p105)
Fusion protein or tag:
Flag
Terminal:
C terminal on backbone
Mutation:
Truncated at the p105 ligation site. It codes for Nfkb1 residues 1-428.
Vector backbone:
cFlag pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5-alpha
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Stephen Smale
Terms and Licenses:
MTA

Comments:

See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20018" in your Materials and Methods section.