Addgene - pMSCV-Flag-hSox2 Plasmid Data

Plasmid 20073: pMSCV-Flag-hSox2

Gene/insert name:
hSox2
Alt name:
SRY (sex determining region Y)-box 2
Insert size:
1000
Species:
H. sapiens (human)
Entrez Gene:
SOX2 (ANOP3, MCOPS3)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pMSCV
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5111
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
ClaI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE-5 List of Sequencing Primers
3' sequencing primer:
MSCV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Juan Belmonte
Terms and Licenses:
MTA

Comments:

Created by Maria J. Barrero.
cDNA was amplified from ES[4]mRNA.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Aasen et al (Nat Biotechnol. 2008 Nov . 26(11):1276-84. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20073" in your Materials and Methods section.