pJSH-B14
(Plasmid #20145)

• Depositing Lab: Glen Hartman
• Publication: Haudenshield et al Plant Dis. 2011 Mar;95(3):343-352. doi: 10.1094/PDIS-01-10-0023.

Backbone

• Vector backbone: pSC-B
• Backbone manufacturer: Stratagene (now Agilent)
• Backbone size w/o insert (bp): 3469
• Vector type: Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: From Stratagene's "Strataclone" kit, using their "SoloPack Competent Cells" which are endA-, recA-, tonA-, restriction minus, and F-, and resistant to streptomycin.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Artificial sequence
• Alt name: HHIC
• Insert Size (bp): 111
• Mutation: n/a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (blunt, see vendor) (not destroyed)
• 3′ cloning site: None (blunt, see vendor) (not destroyed)
• 5′ sequencing primer: M13-20
• 3′ sequencing primer: M13 Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

May linearize backbone using the single XmnI site.