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DHFR-myc
(Plasmid #20214)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3.1/Zeo(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    DHFR
  • Alt name
    dihydrofolate reductase
  • Alt name
    folA
  • Species
    E. coli
  • Insert Size (bp)
    507
  • Entrez Gene
    folA (a.k.a. b0048, ECK0049, JW0047, tmrA)
  • Tag / Fusion Protein
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DHFR-myc was a gift from Carolyn Bertozzi (Addgene plasmid # 20214)
  • For your References section:

    Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, Cornish VW, Bertozzi CR. J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. 10.1021/ja8037728 PubMed 18788807