Addgene - Vh-HA-2xDHFR Plasmid Data

Plasmid 20218: Vh-HA-2xDHFR

Gene/insert name:
2xDHFR
Alt name:
dihydrofolate reductase
Alt name:
fol A
Alt name:
ECK0049
Insert size:
1185
Species:
E. coli
Entrez Gene:
folA (ECK0049, JW0047, tmrA)
Fusion protein or tag:
Vh
Terminal:
N terminal on insert
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pcDNA3.1(+)
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
NdeI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Carolyn Bertozzi
Terms and Licenses:
MTA

Comments:

murine VH chain signal peptide followed by a HA
tag and two repeats of DHFR

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al (J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20218" in your Materials and Methods section.