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TetO-FUW-OSKM Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: XhoI.

We expected the following band sizes for this digest: 1.45kb, 2kb, 2.6kb and 7.3kb. We see the expected pattern.

Addgene XhoI and SacII diagnostic digests

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: XhoI and SacII.

We expected the following band sizes for the XhoI digest: 1.45kb, 2kb, 2.6kb and 7.3kb. We see the expected pattern.

We expected the following band sizes for the SacII digest: 1.35kb, 1.5kb, 2.8kb and 7.8kb. We see the expected pattern.