Addgene - TetO-FUW-Klf4 Plasmid Data

Plasmid 20322: TetO-FUW-Klf4

Gene/insert name:
Klf4
Insert size:
1651
Species:
M. musculus (mouse)
Entrez Gene:
Klf4 (RP23-322L22.2, EZF, Gklf, Zie)
Vector backbone:
FUW
(Search Vector Database)
Backbone manufacturer:
homemade
Vector type:
Lentiviral
Backbone size w/o insert (bp):
8376
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
AAGACCACCGCACAGCAAGC List of Sequencing Primers
3' sequencing primer:
TTCCGCCGTGGCAATAGGGA
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
TOP10, 37C
High or low copy:
High Copy
Selectable markers:
Zeocin
Sequence: View sequences (2)
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Brambrink et al (Cell Stem Cell. 2008 Feb 7. 2(2):151-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20322" in your Materials and Methods section.