|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20327||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneFUW (Lenti-Lox-Ubi)
- Backbone size w/o insert (bp) 7222
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsTOP10, 37C
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer ACTTTGCAGCCTGAGGGCCA (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FUW-SOK was a gift from Rudolf Jaenisch (Addgene plasmid # 20327 ; http://n2t.net/addgene:20327 ; RRID:Addgene_20327)
For your References section:Reprogramming of murine and human somatic cells using a single polycistronic vector. Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, Jaenisch R. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. 10.1073/pnas.0811426106 PubMed 19109433