Purposebacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20341||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2897
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)681
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRSETa mEos2 was a gift from Loren Looger (Addgene plasmid # 20341 ; http://n2t.net/addgene:20341 ; RRID:Addgene_20341)
For your References section:A bright and photostable photoconvertible fluorescent protein. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL. Nat Methods. 2009 Feb . 6(2):131-3. 10.1038/nmeth.1296 PubMed 19169260