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Purposebacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 20341 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRSETa
- Backbone size w/o insert (bp) 2897
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemEos-2
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SpeciesE coli
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Insert Size (bp)681
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSETa mEos2 was a gift from Loren Looger (Addgene plasmid # 20341 ; http://n2t.net/addgene:20341 ; RRID:Addgene_20341) -
For your References section:
A bright and photostable photoconvertible fluorescent protein. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL. Nat Methods. 2009 Feb . 6(2):131-3. 10.1038/nmeth.1296 PubMed 19169260