Addgene - GST TopBP1 D (aa 1-1013) Plasmid Data

Plasmid 20374: GST TopBP1 D (aa 1-1013)

Gene/insert name:
TopBP1 (1-1013)
Alt name:
TOPBP1
Alt name:
topoisomerase II beta binding protein 1
Insert size:
3039
Species:
H. sapiens (human)
Entrez Gene:
TOPBP1 (TOP2BP1)
Mutation:
amino acids 1-1013
Vector backbone:
pGEX6P1
(Search Vector Database)
Backbone manufacturer:
Amersham
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4900
5' sequencing primer:
pGEX 5' List of Sequencing Primers
3' sequencing primer:
pGEX 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Aziz Sancar
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cooperative activation of the ATR checkpoint kinase by TopBP1 and damaged DNA. Choi et al (Nucleic Acids Res. 2009 Jan 12. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20374" in your Materials and Methods section.