pET28a-EgC*
(Plasmid #20378)

• Depositing Lab: Andrew Lee
• Publication: Hu et al Biochemistry. 2003 Dec 2. 42(47):13856-68.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5367
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Eglin C wt*
• Species: Leech
• Insert Size (bp): 216
• Mutation: F10W
• GenBank ID: X00802

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • egCwt_map
• A portion of this plasmid was derived from a plasmid made by: Dr Marshall Edgell (UNC Microbiology and Immunology)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PDZ2 was amplified out of hPTP1e cDNA and ligated into pET21a between sites NdeI (5') and BamHI (3')

IMPORTANT: Please note that the His tag in this Pet28 vector is NOT in frame with the Eglin C