pGEX Itk TEC
(Plasmid #20711)

• Depositing Lab: Leslie Berg
• Publication: Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30.

Backbone

• Vector backbone: pGEX-2T
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Itk TEC
• Alt name: IL2-inducible T-cell kinase
• Species: M. musculus (mouse)
• Mutation: amino acids 97-174
• Entrez Gene: Itk (a.k.a. Emt, Tcsk, Tsk)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: pGEX 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry