|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20730||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBluescriptII SK
- Backbone size w/o insert (bp) 2970
Vector typeMouse Targeting
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)10324
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
There are a few mismatches between the author's sequence and Addgene's sequence. The discrepancies are in the targeting arms and will not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Col1a-frt-hygro-pA was a gift from Rudolf Jaenisch (Addgene plasmid # 20730 ; http://n2t.net/addgene:20730 ; RRID:Addgene_20730)
For your References section:Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R. Genesis. 2006 Jan . 44(1):23-8. 10.1002/gene.20180 PubMed 16400644