Addgene - pFA6a-6xGLY-Myc-hphMX4 Plasmid Data

Plasmid 20770: pFA6a-6xGLY-Myc-hphMX4

Gene/insert name:
None
Vector backbone:
pFA6a
(Search Vector Database)
Vector type:
PCR-based C-terminal tagging
Backbone size (bp):
2513
Cloning site 5':
PacI
Site destroyed during cloning:
Unknown
Cloning site 3':
PmeI
Site destroyed during cloning:
Unknown
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Hygromycin
Sequence: View sequences (2)
Principal Investigator:
Mark Hochstrasser
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae. Funakoshi et al (Yeast. 2009 Feb 25. 26(3):185-192. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20770" in your Materials and Methods section.