pFA6a-6xGLY-Myc-hphMX4
(Plasmid #20770)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pFA6a
  • Backbone size (bp) 2513
  • Vector type
    PCR-based C-terminal tagging
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (unknown if destroyed)
  • 3′ cloning site PmeI (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-6xGLY-Myc-hphMX4 was a gift from Mark Hochstrasser (Addgene plasmid # 20770)
  • For your References section:

    Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae. Funakoshi M, Hochstrasser M. Yeast. 2009 Feb 25. 26(3):185-192. 10.1002/yea.1658 PubMed 19243080