Addgene - pGSTag Flag p38 gamma Plasmid Data

Plasmid 20818: pGSTag Flag p38 gamma

Gene/insert name:
pGSTag Flag p38 gamma
Alt name:
MapK12
Alt name:
SAPK3
Insert size:
1175
Species:
R. norvegicus (rat)
GenBank ID:
NM_021746
Entrez Gene:
Mapk12 (Sapk3, MGC156834)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pGSTag
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5057
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
Xho 1
Site destroyed during cloning:
No
5' sequencing primer:
pGex 5' List of Sequencing Primers
3' sequencing primer:
pGex 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Roger Davis
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6. Enslen et al (J Biol Chem. 1998 Jan 16. 273(3):1741-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20818" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

20816: pGSTag Flag p38 alpha (agf)

20815: pGSTag Flag p38 alpha