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Plasmid 20859: pET21a-Streptavidin-Dead
  • Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

  • Streptavidin-Dead

  • 411

  • E. Coli

  • Triple mutant of wt Streptavidin N23A,S27D,S45A

  • pET21a
    (Search Vector Database)

  • Bacterial Expression

  • 5443

  • NdeI

  • No

  • HindIII

  • No

  • T7 List of Sequencing Primers

  • T7 term

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • Alice Ting

  • MTA
    EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments: 

Please email tinglab@gmail.com with intended use and/or targets for this plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A monovalent streptavidin with a single femtomolar biotin binding site. Howarth et al (Nat Methods. 2006 Apr . 3(4):267-73. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20859" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only