pET21a-Streptavidin-Dead
(Plasmid #20859)

Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Streptavidin-Dead
  • Species
    E. Coli
  • Insert Size (bp)
    411
  • Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please email tinglab@gmail.com with intended use and/or targets for this plasmid.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a-Streptavidin-Dead was a gift from Alice Ting (Addgene plasmid # 20859)
  • For your References section:

    A monovalent streptavidin with a single femtomolar biotin binding site. Howarth M, Chinnapen DJ, Gerrow K, Dorrestein PC, Grandy MR, Kelleher NL, El-Husseini A, Ting AY. Nat Methods. 2006 Apr . 3(4):267-73. 10.1038/nmeth861 PubMed 16554831