Addgene - pCAG2LMKOSimO Plasmid Data

Plasmid 20866: pCAG2LMKOSimO

Gene/insert name:
c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2-ires-mOrange
Insert size:
6302
Species:
M. musculus (mouse)
GenBank ID:
BC006728 NM_011443 NM_013633 NM_010637
Vector backbone:
pCAG
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6397
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5a
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (5)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Keisuke Kaji
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Kaji et al (Nature. 2009 Mar 1. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20866" in your Materials and Methods section.