pIOM15
(Plasmid #20869)

• Depositing Lab: Carmen Beuzón
• Publication: Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):395-407.

Backbone

• Vector backbone: pKAS32
• Backbone manufacturer: Skorupski, K., Taylor, R.K.
• Backbone size w/o insert (bp): 4400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Streptomycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Strains producing the π protein from λ phage. Ej. S17λpir, CC118λpir.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: aphA::aadA
• Alt name: kanamycin resistance gene interrupted by streptomycin resistance gene
• Species: bacteria
• Insert Size (bp): 3700

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains aadA which confers resistance to streptomycin.