Addgene - BAT-gal Plasmid Data

Plasmid 20889: BAT-gal

Gene/insert name:
7XTCF binding sites, Siamois minimal promoter, NLS-lacz
Insert size:
3700
Species:
X. laevis (frog)
Species:
E. coli
Mutation:
7 multimerized TCF-binding elements cloned upstream of the xSiamois minimal promoter, driving expression of NLS-lacZ (nuclear localized beta-galactosidase)
Vector backbone:
pBluescript
(Search Vector Database)
Vector type:
Mammalian Expression, Mouse Targeting
Backbone size w/o insert (bp):
3000
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T3 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Stefano Piccolo
Terms and Licenses:
MTA

Comments:

Wnt/beta-catenin responsive promoter in mouse tissues (used to derive the BAT-gal mouse strain), Xenopus embryos and human cell lines

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mapping Wnt/beta-catenin signaling during mouse development and in colorectal tumors. Maretto et al (Proc Natl Acad Sci U S A. 2003 Mar 18. 100(6):3299-304. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20889" in your Materials and Methods section.