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pcDNA3.1/opto-a1AR-EYFP
(Plasmid #20947)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20947 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    opto-a1AR
  • Species
    synthetic
  • Insert Size (bp)
    2208

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please see http://www.optogenetics.org

for additional information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/opto-a1AR-EYFP was a gift from Karl Deisseroth (Addgene plasmid # 20947)
  • For your References section:

    Temporally precise in vivo control of intracellular signalling. Airan RD, Thompson KR, Fenno LE, Bernstein H, Deisseroth K. Nature. 2009 Mar 18. ():. 10.1038/nature07926 PubMed 19295515