Addgene - pLenti X2 Puro Empty (w609-1) Plasmid Data

Plasmid 20957: pLenti X2 Puro Empty (w609-1)

Gene/insert name:
no insert
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size (bp):
7480
Cloning site 5':
Sal I
Site destroyed during cloning:
No
Cloning site 3':
Xba I
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37oC
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

The BamHI site on the full map seen here (based on the author's assembled sequence) is not present in the actual plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20957" in your Materials and Methods section.