HOXB6-FLAGA-pSP65
(Plasmid
#20994)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20994 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSP65
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typein vitro trans/trans
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXB6
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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Entrez GeneHOXB6 (a.k.a. HOX2, HOX2B, HU-2, Hox-2.2)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI/SalI (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXB6-FLAGA-pSP65 was a gift from Corey Largman (Addgene plasmid # 20994 ; http://n2t.net/addgene:20994 ; RRID:Addgene_20994) -
For your References section:
Hox homeodomain proteins exhibit selective complex stabilities with Pbx and DNA. Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, Lawrence HJ, Cleary ML, Largman C.. Nucleic Acids Res. 1996 Mar 1;24(5):898-906.Click here to read 10.1093/nar/24.5.898 PubMed 8600458
Map uploaded by the depositor.
