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RCAS(B)-Rheb-Q64L
(Plasmid #21050)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 11689
  • Vector type
    Bacterial Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Ras-homolog enriched in brain
  • Alt name
    Rheb
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    685
  • Mutation
    glutamine 64 to leucine
  • GenBank ID
    AF493921
  • Entrez Gene
    RHEB (a.k.a. RHEB2)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI(A) (not destroyed)
  • 3′ cloning site SfiI(B) (not destroyed)
  • 5′ sequencing primer AGCTCCGCGGGCCACCATGGACTACAAGGATGACGATGACAAGGCGGCCGCGCCGCAGTCCAAG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The creation of RCAS(B)-SfiI and the adapter plasmid pBSFI was described in Aoki et al., PNAS. 1998 Dec 8;95(25):14950-5. The SacII/XbaI fragment was cloned into pBSFI and the Sfi fragment was further cloned into RCAS(B)-SfiI.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS(B)-Rheb-Q64L was a gift from Peter Vogt (Addgene plasmid # 21050)
  • For your References section:

    Constitutively active Rheb induces oncogenic transformation. Jiang H, Vogt PK. Oncogene. 2008 Sep 25. 27(43):5729-40. 10.1038/onc.2008.180 PubMed 18521078