Addgene - RCAS(B)-Rheb-Q64L Plasmid Data

Plasmid 21050: RCAS(B)-Rheb-Q64L

Gene/insert name:
Ras-homolog enriched in brain
Alt name:
Rheb
Insert size:
685
Species:
H. sapiens (human)
GenBank ID:
AF493921
Entrez Gene:
RHEB (RHEB2)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Mutation:
glutamine 64 to leucine
Vector backbone:
pBR322
(Search Vector Database)
Vector type:
Bacterial Expression, Retroviral
Backbone size w/o insert (bp):
11689
Cloning site 5':
SfiI(A)
Site destroyed during cloning:
No
Cloning site 3':
SfiI(B)
Site destroyed during cloning:
No
5' sequencing primer:
AGCTCCGCGGGCCACCATGGACTACAAGGATGACGATGACAAGGCGGCCGCGCCGCAGTCCAAG List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Peter Vogt
Terms and Licenses:
MTA

Comments:

The creation of RCAS(B)-SfiI and the adapter plasmid pBSFI was described in Aoki et al., PNAS. 1998 Dec 8;95(25):14950-5. The SacII/XbaI fragment was cloned into pBSFI and the Sfi fragment was further cloned into RCAS(B)-SfiI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Constitutively active Rheb induces oncogenic transformation. Jiang et al (Oncogene. 2008 Sep 25. 27(43):5729-40. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21050" in your Materials and Methods section.