Addgene - human oct4-GFP Plasmid Data

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Plasmid 21153: human oct4-GFP

Gene/insert name:
hOCT4-GFP
Alt name:
hCol1a1 targeting arms
Insert size:
120006
Species:
H. sapiens (human)
Vector backbone:
BluescriptKSII
(Search Vector Database)
Backbone manufacturer:
stratagene
Vector type:
Mammalian Expression
Vector type:
human targeting
Backbone size w/o insert:
2381
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CGATAAGCTTGGTACCGAGC List of Sequencing Primers
3' sequencing primer:
CCAACACACTATTGCAATGAAA
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
Growth instructions:
XL1blue
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Nanoparticles for gene transfer to human embryonic stem cell colonies. Green et al (Nano Lett. 2008 Oct . 8(10):3126-30. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21153" in your Materials and Methods section.