|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21164||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression, Lentiviral ; reprogramming
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameKlf4, cMyc
SpeciesH. sapiens (human)
Insert Size (bp)2733
GenBank IDNM_004235.2 NM_002467
Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer EF1a_for (Common Sequencing Primers)
After lentivirus infection, Klf4 and cMyc are coexpressed by CMV promoter and IRES intron. Infections with this lentivirus, when used with pSIN4-EF2-O2S and pSIN4-EF2-N2L have a significantly greater reprogramming efficiency than our original 4 lentiviruses.
NOTE: Addgene's quality control sequence found a K269T in Klf4. This mutation is not known to affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSIN4-CMV-K2M was a gift from James Thomson (Addgene plasmid # 21164 ; http://n2t.net/addgene:21164 ; RRID:Addgene_21164)
For your References section:Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences. Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. Science. 2009 Mar 26. 10.1126/science.1172482 PubMed 19325077