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Plasmid 21165: pOTF3-AK (clone3)
  • POUF5F1 (genomic DNA)

  • Oct4

  • 13500

  • H. sapiens (human)

  • AC006047

  • POU5F1 (DADB-104B20.2, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)

  • NeoR, EGFP, and SV40 polyA are between the two genomic sequences (one section is 1604 bp and one is 9022).

  • pBluescript SK (-)
    (Search Vector Database)

  • Stratagene

  • Cre/Lox ; homologous recombination

  • 2900

  • SalI

  • No

  • NotI

  • No

  • T7 List of Sequencing Primers

  • pLac

  • Ampicillin

  • DH5alpha

  • 37

  • Low Copy

  • Neomycin

  • View sequences (2)
  • View map

  • James Thomson

  • UBMTA

Comments: 

The gene-targeting vector was constructed by insertion of an IRES-EGFP, and IRES-Neo, and an SV40 polyadenylation sequenc (aprox 3.2kb) into the 3' untranslated region of the fifth exon of the human POU5F1 gene

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Homologous recombination in human embryonic stem cells. Zwaka et al (Nat Biotechnol. 2003 Mar . 21(3):319-21. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21165" in your Materials and Methods section.