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pOTF3-AK (clone3)
(Plasmid #21165)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBluescript SK (-)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Cre/Lox ; homologous recombination
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    POUF5F1 (genomic DNA)
  • Alt name
    Oct4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    13500
  • Mutation
    NeoR, EGFP, and SV40 polyA are between the two genomic sequences (one section is 1604 bp and one is 9022).
  • GenBank ID
    AC006047
  • Entrez Gene
    POU5F1 (a.k.a. DADB-104B20.2, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer pLac
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The gene-targeting vector was constructed by insertion of an IRES-EGFP, and IRES-Neo, and an SV40 polyadenylation sequenc (aprox 3.2kb) into the 3' untranslated region of the fifth exon of the human POU5F1 gene

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTF3-AK (clone3) was a gift from James Thomson (Addgene plasmid # 21165)
  • For your References section:

    Homologous recombination in human embryonic stem cells. Zwaka TP, Thomson JA. Nat Biotechnol. 2003 Mar . 21(3):319-21. 10.1038/nbt788 PubMed 12577066