Addgene - T/Brachyury-eGFP Rex Neo Plasmid Data

Plasmid 21222: T/Brachyury-eGFP Rex Neo

Gene/insert name:
Brachyury promoter
Insert size:
644
Species:
M. musculus (mouse)
Fusion protein or tag:
eGFP
Terminal:
C terminal on backbone
Vector backbone:
SIN18
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
7838
Cloning site 5':
claI
Site destroyed during cloning:
No
Cloning site 3':
ageI
Site destroyed during cloning:
No
5' sequencing primer:
CTTGATGCCGTTCTTCTGCTT - 3' sequencing only List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers, other:
Neomycin resistanct marker driven by Rex-1 promoter
Sequence: View sequences (2)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Mark Mercola
Terms and Licenses:
MTA

Comments:

There are several mismatches between depositor's and Addgene sequence. These mutations do not affect eGFP expression.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes. Kita-Matsuo et al (PLoS ONE. 2009 . 4(4):e5046. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21222" in your Materials and Methods section.